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mcd99 In contrast, the anti-mCD99 antibody recognizes the mCD99-Fc used to immunize the rabbits (arrowhead, running at the expected M r of ∼ 75 kD). Display full size. Fure 4. Clones expressing mCD99 were harvested either by trypsin or using 10 mM EDTA in Hanks’ Balanced Salt Solution (HBSS). Cells were washed twice in PBS and lysated with 0.4% NP40

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